RQ8111

antibody from NSJ Bioreagents

Targeting: PPP3CA CALN, CALNA, CNA1, PPP2B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8111

Provider

NSJ Bioreagents

Product name

Calcineurin Antibody / PPP3CA

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the PPP3CA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HeLa, 2) human U-87 MG and 3) rat C6 cell lysate with PPP3CA antibody. Predicted molecular weight: 51-59 kDa, ~32 kDa (multiple isoforms).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with PPP3CA antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human SiHa cells with PPP3CA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP3CA antibody.