MAB2839

antibody from R&D Systems

Targeting: PPP3CA CALN, CALNA, CNA1, PPP2B

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2839

Provider

R&D Systems

Product name

Human/Mouse/Rat Calcineurin A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat Calcineurin A.

Reactivity

Human, Mouse, Rat

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

302202

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Insulin dysfunction induces in vivo tau hyperphosphorylation through distinct mechanisms.

Planel E, Tatebayashi Y, Miyasaka T, Liu L, Wang L, Herman M, Yu WH, Luchsinger JA, Wadzinski B, Duff KE, Takashima A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Dec 12;27(50):13635-48

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat Calcineurin A by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, TS1 mouse helper T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat Calcineurin A Monoclonal Antibody (Catalog # MAB2839) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Human Calcineurin (Catalog # 3160-CA) (1 ng) was included. A specific band for Calcineurin A was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.