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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- 20127-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM49B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGNLLKVLT CTDLEQGPNF FLDFENAQPT ESEKEIYNQV NVVLKDAEGI LEDLQSYRGA GHEIREAIQH PADEKLQEKA WGAVVPLVGK LKKFYEFSQR LEAALRGLLG ALTSTPYSPT QHLEREQALA KQFAEILHFT LRFDELKMTN PAIQNDFSYY RRTLSRMRIN NVPAEGENEV NNELANRMSL FYAEATPMLK TLSDATTKFV SENKNLPIEN TTDCLSTMAS VCRVMLETPE YRSRFTNEET VSFCLRVMVG VIILYDHVHP VGAFAKTSKI DMKGCIKVLK DQPPNSVEGL LNALRYTTKH LNDETTSKQI KSMLQ (1-324 aa encoded by BC003599)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 20127-1-AP (FAM49B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using FAM49B antibody 20127-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 20127-1-AP (FAM49B antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FAM49B (IP:20127-1-AP, 4ug; Detection:20127-1-AP 1:500) with mouse brain tissue lysate 4000ug.
