PA5-142085

antibody from Invitrogen Antibodies

Targeting: TBC1D10A AC004997.C22.2, EPI64, TBC1D10

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142085

Provider

Invitrogen Antibodies

Product name

TBC1D10A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:2,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TBC1D10A in MCF7 cells using TBC1D10A Polyclonal Antibody (Product # PA5-142085). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic/Plasma Membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TBC1D10A in paraffin embedded Human Prostate using TBC1D10A Polyclonal Antibody (Product # PA5-142085) at 4.75 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TBC1D10A in paraffin embedded Human Liver using TBC1D10A Polyclonal Antibody (Product # PA5-142085) at 4.75 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TBC1D10A in HEK293 cells (blue line) using TBC1D10A Polyclonal Antibody (Product # PA5-142085). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.