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LearnNB120-2864
antibody from Novus Biologicals
Targeting: CACNA2D1
CACNA2, CACNL2A, LINC01112, lncRNA-N3, MHS3
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NB120-2864 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-2864, RRID:AB_789591
- Product name
- Mouse Monoclonal CACNA2D1 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. Detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from skeletal and cardiac muscle. Detects a band at 220 kDa under non-reducing and a 143 kDa band under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with NB120-2864 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Prognostic and clinicopathological significance of Cacna2d1 expression in epithelial ovarian cancers: a retrospective study.
Gabapentinoid Insensitivity after Repeated Administration is Associated with Down-Regulation of the α(2)δ-1 Subunit in Rats with Central Post-Stroke Pain Hypersensitivity.
Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes.
Functional vitamin D receptor (VDR) in the t-tubules of cardiac myocytes: VDR knockout cardiomyocyte contractility.
Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle.
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle.
Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells.
Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells.
Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads.
Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads.
Yu D, Holm R, Goscinski MA, Trope CG, Nesland JM, Suo Z
American journal of cancer research 2016;6(9):2088-2097
American journal of cancer research 2016;6(9):2088-2097
Gabapentinoid Insensitivity after Repeated Administration is Associated with Down-Regulation of the α(2)δ-1 Subunit in Rats with Central Post-Stroke Pain Hypersensitivity.
Yang Y, Yang F, Yang F, Li CL, Wang Y, Li Z, Lu YF, Yu YQ, Fu H, He T, Sun W, Wang RR, Chen J
Neuroscience bulletin 2016 Feb;32(1):41-50
Neuroscience bulletin 2016 Feb;32(1):41-50
Decrease in the density of t-tubular L-type Ca2+ channel currents in failing ventricular myocytes.
Horiuchi-Hirose M, Kashihara T, Nakada T, Kurebayashi N, Shimojo H, Shibazaki T, Sheng X, Yano S, Hirose M, Hongo M, Sakurai T, Moriizumi T, Ueda H, Yamada M
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H978-88
American journal of physiology. Heart and circulatory physiology 2011 Mar;300(3):H978-88
Functional vitamin D receptor (VDR) in the t-tubules of cardiac myocytes: VDR knockout cardiomyocyte contractility.
Tishkoff DX, Nibbelink KA, Holmberg KH, Dandu L, Simpson RU
Endocrinology 2008 Feb;149(2):558-64
Endocrinology 2008 Feb;149(2):558-64
Subcellular localization of the delayed rectifier K(+) channels KCNQ1 and ERG1 in the rat heart.
Rasmussen HB, Møller M, Knaus HG, Jensen BS, Olesen SP, Jørgensen NK
American journal of physiology. Heart and circulatory physiology 2004 Apr;286(4):H1300-9
American journal of physiology. Heart and circulatory physiology 2004 Apr;286(4):H1300-9
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle.
Protasi F, Franzini-Armstrong C, Allen PD
The Journal of cell biology 1998 Feb 23;140(4):831-42
The Journal of cell biology 1998 Feb 23;140(4):831-42
Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle.
Protasi F, Franzini-Armstrong C, Allen PD
The Journal of cell biology 1998 Feb 23;140(4):831-42
The Journal of cell biology 1998 Feb 23;140(4):831-42
Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells.
Morton ME, Cassidy TN, Froehner SC, Gilmour BP, Laurens RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1994 Aug;8(11):884-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1994 Aug;8(11):884-8
Alpha 1 and alpha 2 Ca2+ channel subunit expression in human neuronal and small cell carcinoma cells.
Morton ME, Cassidy TN, Froehner SC, Gilmour BP, Laurens RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1994 Aug;8(11):884-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1994 Aug;8(11):884-8
Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads.
Flucher BE, Morton ME, Froehner SC, Daniels MP
Neuron 1990 Sep;5(3):339-51
Neuron 1990 Sep;5(3):339-51
Localization of the alpha 1 and alpha 2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triads.
Flucher BE, Morton ME, Froehner SC, Daniels MP
Neuron 1990 Sep;5(3):339-51
Neuron 1990 Sep;5(3):339-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Primary antibody dilution- 1:1000 for 2 Hours at RT, in blocking buffer, 5% Milk in TBS-0.05% Tween : Blocking Overnight at +4 degree C. Secondary antibody : 1:2000 in blocking buffer, 1 hour at RT. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized mouse skeletal muscle tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized human skeletal muscle tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of SH-SY5Y cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of C6 cells compared to an isotype control (blue).
