Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB2140 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Vasorin/SLIT-like 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Vasorin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Vasorin/ATIA Promotes Cigarette Smoke-Induced Transformation of Human Bronchial Epithelial Cells by Suppressing Autophagy-Mediated Apoptosis.
Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Chen W, Wang Q, Xu X, Saxton B, Tessema M, Leng S, Choksi S, Belinsky SA, Liu ZG, Lin Y
Translational oncology 2020 Jan;13(1):32-41
Translational oncology 2020 Jan;13(1):32-41
Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Gunawardana CG, Kuk C, Smith CR, Batruch I, Soosaipillai A, Diamandis EP
Journal of proteome research 2009 Oct;8(10):4705-13
Journal of proteome research 2009 Oct;8(10):4705-13
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Vasorin/SLIT-like 2 by Western Blot. Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT-like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT-like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.