Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB2140 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Vasorin/SLIT-like 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Vasorin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q6EMK4
- Isotype
- IgG
- Antibody clone number
- 227526
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
Gunawardana CG, Kuk C, Smith CR, Batruch I, Soosaipillai A, Diamandis EP
Journal of proteome research 2009 Oct;8(10):4705-13
Journal of proteome research 2009 Oct;8(10):4705-13
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Vasorin/SLIT-like 2 by Western Blot. Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT-like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT-like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.