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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- BSM-52241R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Hsp70 Monoclonal Antibody (2G2)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody reacts with the alpha-synuclein protein. This antibody does not cross-react with beta- or gamma-synuclein or synoretin. During development reactivity was confirmed with human. Reactivity with other species has not been tested.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2G2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Lane 1: MCF-7; Lane 2: HCT116; Probed with Hsp70 (2G2) Monoclonal Antibody (bsm-52241R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF(ICC) staining with Hsp70 (2G2) Monoclonal Antibody (bsm-52241R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with Hsp70 (2G2) Monoclonal Antibody (bsm-52241R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Hsp70 (2G2) Monoclonal Antibody (bsm-52241R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Hsp70 (2G2) Monoclonal Antibody (bsm-52241R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabeled control (cells with out incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
