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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52692PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52692PU-N, RRID:AB_11144177
- Product name
- anti C18orf8 (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 40-74aa) of human C18orf8/MIC1.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 2.0 mg/mL
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using C18orf8/MIC1 antibody Cat.-No. AP52692PU-N (N-term). This demonstrates the antibody detected the MIC1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MDA-MBA231 cells using C18orf8/MIC1 antibody Cat.-No. AP52692PU-N (N-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) using C18orf8/MIC1 antibody Cat.-No. AP52692PU-N (N-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
