Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-71579 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIC-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Elevated circulating macrophage inhibitory cytokine 1 is a biological marker for the diagnosis and prognosis of osteosarcoma.
Song X, Tian L, Quan Y, Sun D, Zhang L
Experimental and therapeutic medicine 2018 Dec;16(6):4803-4809
Experimental and therapeutic medicine 2018 Dec;16(6):4803-4809
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIC1 in MDA-MB231 cell lysate using 35 µg of protein per lane. Samples were incubated with MIC1 polyclonal antibody (Product # PA5-71579).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MIC1 in MDA-MBA231 cells using confocal microscopy. Samples were incubated with MIC1 H1 polyclonal antibody (Product # PA5-71579) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) as a secondary antibody. Nuclei were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MIC1 in HeLa cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with MIC1 polyclonal antibody (Product # PA5-71579) followed by a FITC conjugated goat anti-rabbit secondary antibody.