AF2758

antibody from Novus Biologicals

Targeting: DEFB4A DEFB-2, DEFB102, DEFB2, DEFB4, HBD-2, SAP1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2758

Provider

Novus Biologicals

Product name

Goat Polyclonal beta-Defensin 4/2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human beta-Defensin 4/2 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Inflammatory cytokines and antimicrobial peptides in acquired heart diseases.

Vartina E, Pilmane M, Lacis R
Histology and histopathology 2019 Aug;34(8):889-897

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Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.

Haisma EM, Rietveld MH, de Breij A, van Dissel JT, El Ghalbzouri A, Nibbering PH
PloS one 2013;8(12):e82800

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A novel organotypic dento-epithelial culture model: effect of Fusobacterium nucleatum biofilm on B-defensin-2, -3, and LL-37 expression.

Gursoy UK, Pöllänen M, Könönen E, Uitto VJ
Journal of periodontology 2012 Feb;83(2):242-7

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

beta-Defensin 4/2 in Human Kidney Array. beta-Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human beta-Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

beta-Defensin 4/2 in Human Lung. beta-Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human lung using Human beta-Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.