Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF265 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Angiogenin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Angiogenin in direct ELISAs and Western blots.
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references An essential role of PRMT1-mediated EGFR methylation in EGFR activation by ribonuclease 5.
Pleiotropic activity of systemically delivered angiogenin in the SOD1G93A mouse model.
Angiogenin/Ribonuclease 5 Is an EGFR Ligand and a Serum Biomarker for Erlotinib Sensitivity in Pancreatic Cancer.
Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C.
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
A neuroprotective role for angiogenin in models of Parkinson's disease.
Wang WJ, Hsu JM, Wang YN, Lee HH, Yamaguchi H, Liao HW, Hung MC
American journal of cancer research 2019;9(1):180-185
American journal of cancer research 2019;9(1):180-185
Pleiotropic activity of systemically delivered angiogenin in the SOD1G93A mouse model.
Crivello M, O'Riordan SL, Woods I, Cannon S, Halang L, Coughlan KS, Hogg MC, Lewandowski SA, Prehn JHM
Neuropharmacology 2018 May 1;133:503-511
Neuropharmacology 2018 May 1;133:503-511
Angiogenin/Ribonuclease 5 Is an EGFR Ligand and a Serum Biomarker for Erlotinib Sensitivity in Pancreatic Cancer.
Wang YN, Lee HH, Chou CK, Yang WH, Wei Y, Chen CT, Yao J, Hsu JL, Zhu C, Ying H, Ye Y, Wang WJ, Lim SO, Xia W, Ko HW, Liu X, Liu CG, Wu X, Wang H, Li D, Prakash LR, Katz MH, Kang Y, Kim M, Fleming JB, Fogelman D, Javle M, Maitra A, Hung MC
Cancer cell 2018 Apr 9;33(4):752-769.e8
Cancer cell 2018 Apr 9;33(4):752-769.e8
Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C.
Selitsky SR, Baran-Gale J, Honda M, Yamane D, Masaki T, Fannin EE, Guerra B, Shirasaki T, Shimakami T, Kaneko S, Lanford RE, Lemon SM, Sethupathy P
Scientific reports 2015 Jan 8;5:7675
Scientific reports 2015 Jan 8;5:7675
Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.
Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB
PloS one 2012;7(6):e38798
PloS one 2012;7(6):e38798
A neuroprotective role for angiogenin in models of Parkinson's disease.
Steidinger TU, Standaert DG, Yacoubian TA
Journal of neurochemistry 2011 Feb;116(3):334-41
Journal of neurochemistry 2011 Feb;116(3):334-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Rat Angiogenin by Western Blot. Western blot shows human plasma and rat plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Rat Angiogenin by Simple WesternTM. Simple Western lane view shows human plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Angiogenin at approximately 20 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.