AF265

antibody from Novus Biologicals

Targeting: ANG RAA1, RNASE5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF265

Provider

Novus Biologicals

Product name

Goat Polyclonal Angiogenin Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Angiogenin in direct ELISAs and Western blots.

Reactivity

Human, Rat

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

An essential role of PRMT1-mediated EGFR methylation in EGFR activation by ribonuclease 5.

Wang WJ, Hsu JM, Wang YN, Lee HH, Yamaguchi H, Liao HW, Hung MC
American journal of cancer research 2019;9(1):180-185

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Pleiotropic activity of systemically delivered angiogenin in the SOD1^G93A mouse model.

Crivello M, O'Riordan SL, Woods I, Cannon S, Halang L, Coughlan KS, Hogg MC, Lewandowski SA, Prehn JHM
Neuropharmacology 2018 May 1;133:503-511

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Angiogenin/Ribonuclease 5 Is an EGFR Ligand and a Serum Biomarker for Erlotinib Sensitivity in Pancreatic Cancer.

Wang YN, Lee HH, Chou CK, Yang WH, Wei Y, Chen CT, Yao J, Hsu JL, Zhu C, Ying H, Ye Y, Wang WJ, Lim SO, Xia W, Ko HW, Liu X, Liu CG, Wu X, Wang H, Li D, Prakash LR, Katz MH, Kang Y, Kim M, Fleming JB, Fogelman D, Javle M, Maitra A, Hung MC
Cancer cell 2018 Apr 9;33(4):752-769.e8

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Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C.

Selitsky SR, Baran-Gale J, Honda M, Yamane D, Masaki T, Fannin EE, Guerra B, Shirasaki T, Shimakami T, Kaneko S, Lanford RE, Lemon SM, Sethupathy P
Scientific reports 2015 Jan 8;5:7675

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Mucin Muc2 deficiency and weaning influences the expression of the innate defense genes Reg3β, Reg3γ and angiogenin-4.

Burger-van Paassen N, Loonen LM, Witte-Bouma J, Korteland-van Male AM, de Bruijn AC, van der Sluis M, Lu P, Van Goudoever JB, Wells JM, Dekker J, Van Seuningen I, Renes IB
PloS one 2012;7(6):e38798

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A neuroprotective role for angiogenin in models of Parkinson's disease.

Steidinger TU, Standaert DG, Yacoubian TA
Journal of neurochemistry 2011 Feb;116(3):334-41

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Rat Angiogenin by Western Blot. Western blot shows human plasma and rat plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiogenin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Rat Angiogenin by Simple Western^TM. Simple Western lane view shows human plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Angiogenin at approximately 20 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Rat Angiogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF265) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.