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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-97305 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNASE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KPPQFTWAQW FETQHINMTS QQCTNAMQVI NNYQRRCKNQ NTFLLTTFAN VVNVCGNPNM TCPSNKTRKN CHHSGSQVPL IHCNLTTPSP QNISNCRYAQ TPANMFYIVA CDNRDQRRDP PQYPVVPVHL DRII; Positive Samples: K-562, HL-60, Mouse liver; Cellular Location: Cytoplasmic granule, Lysosome
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.52 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Immune Sensing of Synthetic, Bacterial, and Protozoan RNA by Toll-like Receptor 8 Requires Coordinated Processing by RNase T2 and RNase 2.
Ostendorf T, Zillinger T, Andryka K, Schlee-Guimaraes TM, Schmitz S, Marx S, Bayrak K, Linke R, Salgert S, Wegner J, Grasser T, Bauersachs S, Soltesz L, Hübner MP, Nastaly M, Coch C, Kettwig M, Roehl I, Henneke M, Hoerauf A, Barchet W, Gärtner J, Schlee M, Hartmann G, Bartok E
Immunity 2020 Apr 14;52(4):591-605.e6
Immunity 2020 Apr 14;52(4):591-605.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using RNASE2 Polyclonal antibody (Product # PA5-97305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RNASE2 in extracts of various cell lines using RNASE2 Polyclonal Antibody (Product # PA5-97305) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
