HPA059157

antibody from Atlas Antibodies

Targeting: WNK1 HSAN2, HSN2, PPP1R167, PRKWNK1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA059157

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA059157, RRID:AB_2683935

Product name

Anti-WNK1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PPFPAPSLTQSQQPLEDLDAQLRRTLSPEMITVTS
AVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLAT
SSGAGVFKMGRFQVSVAADGA


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human esophagus and pancreas tissues using Anti-WNK1 antibody. Corresponding WNK1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN