PA5-28382

antibody from Invitrogen Antibodies

Targeting: WNK1 HSAN2, HSN2, PPP1R167, PRKWNK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28382

Provider

Invitrogen Antibodies

Product name

WNK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2.

Concentration

1.18 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of WNK1 using 30 µg of H1299 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a WNK1 polyclonal antibody (Product # PA5-28382) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNK1 was performed by separating 30 µg of whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using WNK1 Polyclonal Antibody (Product # PA5-28382). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 Polyclonal Antibody (Product # PA5-28382) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of WNK1 was performed by separating 30 µg of whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of WNK1 in methanol-fixed HeLa cells using a WNK1 polyclonal antibody (Product # PA5-28382) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of WNK1 was performed in paraffin-embedded human lung adenocarcinoma tissue using WNK1 Polyclonal Antibody (Product # PA5-28382) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1 (Product # PA5-28382) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WNK1 Polyclonal Antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. WNK1 stained by WNK1 Polyclonal Antibody (Product # PA5-28382) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.