PA5-89003

antibody from Invitrogen Antibodies

Targeting: MTMR9 C8orf9, DKFZp434K171, LIP-STYX, MTMR8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-89003

Provider

Invitrogen Antibodies

Product name

Mtmr9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MEFAELIKTP RVDNVVLHRP FYPAVEGTLC LTGHHLILSS RQDNTEELWL LHSNIDAIDK RFVGSLGTII IKCKDFRIIQ LDIPGMEECL NIASSIEALS TLDSITLMYP FFYRPMFEVI EDGWHSFLPE QEFELYSSAT SEWRLSYVNK EFAVCPSYPP IVTVPKSIDD EALRKVATFR HGGRFPVLSY YHKKNGMVIM RSGQPLTGTN GRRCKEDEKL INATLRAGKR GYIIDTRSLN VAQQTRAKGG; Positive Samples: HepG2, HeLa, Jurkat, A-549, Mouse brain, Rat brain, Rat lung; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.35 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using MTMR9 Polyclonal antibody (Product # PA5-89003) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MTMR9 in extracts of various cell lines using Mtmr9 Polyclonal Antibody (Product # PA5-89003) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MTMR9 in extracts of various cell lines using Mtmr9 Polyclonal Antibody (Product # PA5-89003) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MTMR9 was performed in HeLa cells using Mtmr9 Polyclonal Antibody (Product # PA5-89003) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MTMR9 was performed in L929 cells using Mtmr9 Polyclonal Antibody (Product # PA5-89003) at a dilution of 1:100. Blue: DAPI for nuclear staining.