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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3822 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3822, RRID:AB_1582068
- Product name
- ZNF219 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ZNF219.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins.
Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins.
Paces-Fessy M, Boucher D, Petit E, Paute-Briand S, Blanchet-Tournier MF
The Biochemical journal 2004 Mar 1;378(Pt 2):353-62
The Biochemical journal 2004 Mar 1;378(Pt 2):353-62
Identification of the DNA binding specificity of the human ZNF219 protein and its function as a transcriptional repressor.
Sakai T, Hino K, Wada S, Maeda H
DNA research : an international journal for rapid publication of reports on genes and genomes 2003 Aug 31;10(4):155-65
DNA research : an international journal for rapid publication of reports on genes and genomes 2003 Aug 31;10(4):155-65
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF219 polyclonal antibody (Cat # PAB3822) in 293 cell line lysates (35 ug/lane).ZNF219 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testisreacted with ZNF219 polyclonal antibody (Cat # PAB3822), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
