NBP1-22439

antibody from Novus Biologicals

Targeting: CACNA1C CACH2, CACN2, CACNL1A1, Cav1.2, CCHL1A1, LQT8, TS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-22439

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-22439, RRID:AB_1659985

Product name

Mouse Monoclonal Cav1.2 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 240kDa (varies with cell background due to glycosylation).

Reactivity

Human, Mouse, Rat, Hamster, Rabbit

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

What constitutes the fat signal detected by MRI in the spine of patients with ankylosing spondylitis? A prospective study based on biopsies obtained during planned spinal osteotomy to correct hyperkyphosis or spinal stenosis.

Baraliakos X, Boehm H, Bahrami R, Samir A, Schett G, Luber M, Ramming A, Braun J
Annals of the rheumatic diseases 2019 Sep;78(9):1220-1225

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Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects.

Penney J, Seo J, Kritskiy O, Elmsaouri S, Gao F, Pao PC, Su SC, Tsai LH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Sep 6;37(36):8655-8666

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PI3Kγ inhibition reduces blood pressure by a vasorelaxant Akt/L-type calcium channel mechanism.

Carnevale D, Vecchione C, Mascio G, Esposito G, Cifelli G, Martinello K, Landolfi A, Selvetella G, Grieco P, Damato A, Franco E, Haase H, Maffei A, Ciraolo E, Fucile S, Frati G, Mazzoni O, Hirsch E, Lembo G
Cardiovascular research 2012 Jan 1;93(1):200-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cav1.2 Antibody (S57-46) [NBP1-22439] - analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 . Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cav1.2 Antibody (S57-46) [NBP1-22439] - analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57-46 . Tissue: INS-1E cells. Species: Rat. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody at 1:200. Courtesy of: Merrie Mosedale.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57-46) [NBP1-22439] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cav1.2 Antibody (S57-46) [NBP1-22439] - Human hippocampus tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.