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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [4]
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- Product number
- RQ7287 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TER ATPase Antibody / VCP / Valosin-containing protein
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TER ATPase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat lung, 3) rat stomach, 4) rat C6, 5) mouse brain, 6) mouse lung, 7) mouse stomach and 8) mouse RAW264.7 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human SH-SY5Y, 3) human MCF7, 4) human A431, 5) monkey COS-7, 6) human K562, 7) human placenta and 8) human HepG2 cell lysate with TER ATPase antibody. Expected molecular weight: 89-97 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat colon tissue with TER ATPase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MCF7 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of mouse EL-4 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of rat C6 cells with TER ATPase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TER ATPase antibody.
