PA5-21962

antibody from Invitrogen Antibodies

Targeting: RAB2B FLJ14824

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21962

Provider

Invitrogen Antibodies

Product name

RAB2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HepG2, mouse brain.

Concentration

0.83 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAB2B using 50 µg of mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RAB2B polyclonal antibody (Product # PA5-21962) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RAB2B Polyclonal Antibody (Product # PA5-21962). Sample (30 µg whole cell lysate). A: Hep G2. 12% SDS PAGE. RAB2B Polyclonal Antibody (Product # PA5-21962) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RAB2B Polyclonal Antibody (Product # PA5-21962). Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB2B Polyclonal Antibody (Product # PA5-21962) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAB2B in paraformaldehyde-fixed HeLa cells using a RAB2B polyclonal antibody (Product # PA5-21962) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAB2B (Product # PA5-21962) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAB2B Polyclonal Antibody detects RAB2B protein at cytoplasm and membrane on human liver carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded liver carcinoma. RAB2B Polyclonal Antibody (Product # PA5-21962) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.