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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3267 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3267, RRID:AB_1575049
- Product name
- GAK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GAK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells.
Structure, expression, and chromosomal localization of human GAK.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA, Mammalian Gene Collection Program Team
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16899-903
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16899-903
Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells.
Greener T, Zhao X, Nojima H, Eisenberg E, Greene LE
The Journal of biological chemistry 2000 Jan 14;275(2):1365-70
The Journal of biological chemistry 2000 Jan 14;275(2):1365-70
Structure, expression, and chromosomal localization of human GAK.
Kimura SH, Tsuruga H, Yabuta N, Endo Y, Nojima H
Genomics 1997 Sep 1;44(2):179-87
Genomics 1997 Sep 1;44(2):179-87
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GAK (arrow) using GAK polyclonal antibody (Cat # PAB3267).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GAK polyclonal antibody (Cat # PAB3267) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
