PA5-28443

antibody from Invitrogen Antibodies

Targeting: DLC1 ARHGAP7, DLC-1, HP, p122-RhoGAP, STARD12

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28443

Provider

Invitrogen Antibodies

Product name

DLC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: H1299, HeLaS3, HepG2, Molt-4, Raji.

Concentration

0.84 mg/mL

References

Submitted references

Aberrant DNA methylation of alternative promoter of DLC1 isoform 1 in meningiomas.

Bujko M, Kober P, Rusetska N, Wakuła M, Goryca K, Grecka E, Matyja E, Neska J, Mandat T, Bonicki W, Siedlecki JA
Journal of neuro-oncology 2016 Dec;130(3):473-484

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DLC1 Polyclonal Antibody (Product # PA5-28443). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 7.5% SDS PAGE. DLC1 Polyclonal Antibody (Product # PA5-28443) diluted at 1:1500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Pheochromocytoma xenograft, using DLC1 (Product # PA5-28443) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 a Examples of immunohistochemical staining of DLC1-v1/-v3 protein expression in meningiomas. Magnification x400. b Comparison of DNA methylation levels in meningiomas with high/moderate DLC1 expression and meningiomas with none/poor DLC1 expression. c Comparing the relative expression levels of DLC1v1 and DLC1v3 measured by 220511_s_at probeset (HG U133A Array, Affymetrix), GSE43290 dataset. Horizontal lines represent the mean value