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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6598 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal DGK-theta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha , -beta , -gamma , -epsilon , -iota , -kappa , or -zeta is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human, Mouse, and Rat DGK-theta by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, TF-1 human erythroleukemic cell line, HEK293 human embryonic kidney cell line, mouse brain tissue, and rat brain tissue. PVDF Membrane was probed with 1 µg/mL of Human DGK-theta Monoclonal Antibody (Catalog # MAB6598) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-theta at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
