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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00006297-M10 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006297-M10, RRID:AB_606979
- Product name
- SALL2 monoclonal antibody (M10), clone 3F7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SALL2.
- Antigen sequence
MAHESERSSRLGVPCGEPAELGGDASEEDHPQVCA
KCCAQFTDPTEFLAHQNACSTDPPVMVIIGGQENP
NNSSASSEPRPEGHNNPQVMDTEHSNPPDSGSSVP
TDPTWGPERRGEESPGHFLVAATEPVCGIPVKWPA
HEALEFQLHLHYHSKPGPTSAVWPRNCGWEGASNN
GIQGSQGEDSPPPISASCTQGSA- Isotype
- IgG
- Antibody clone number
- 3F7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SALL2 expression in transfected 293T cell line by SALL2 monoclonal antibody (M10), clone 3F7.Lane 1: SALL2 transfected lysate(20.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SALL2 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SALL2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 1.2 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
