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LearnH00007004-M01
antibody from Novus Biologicals
Targeting: TEAD4
EFTR-2, RTEF-1, TCF13L1, TEF-3, TEFR-1
Western blot ELISA
Immunocytochemistry Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00007004-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007004-M01, RRID:AB_548608
- Product name
- Mouse Monoclonal TEAD4 Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. TEAD4 - TEA domain family member 4
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Epigenetic silencing of miR-1271 enhances MEK1 and TEAD4 expression in gastric cancer.
Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function.
Mechanism of action of a WWTR1(TAZ)-CAMTA1 fusion oncoprotein.
Integrative genomics analysis reveals the multilevel dysregulation and oncogenic characteristics of TEAD4 in gastric cancer.
Induction of a trophoblast-like phenotype by hydralazine in the p19 embryonic carcinoma cell line.
Transcription factor TEAD4 regulates expression of myogenin and the unfolded protein response genes during C2C12 cell differentiation.
Lim B, Kim HJ, Heo H, Huh N, Baek SJ, Kim JH, Bae DH, Seo EH, Lee SI, Song KS, Kim SY, Kim YS, Kim M
Cancer medicine 2018 Jul;7(7):3411-3424
Cancer medicine 2018 Jul;7(7):3411-3424
Common and Distinctive Functions of the Hippo Effectors Taz and Yap in Skeletal Muscle Stem Cell Function.
Sun C, De Mello V, Mohamed A, Ortuste Quiroga HP, Garcia-Munoz A, Al Bloshi A, Tremblay AM, von Kriegsheim A, Collie-Duguid E, Vargesson N, Matallanas D, Wackerhage H, Zammit PS
Stem cells (Dayton, Ohio) 2017 Aug;35(8):1958-1972
Stem cells (Dayton, Ohio) 2017 Aug;35(8):1958-1972
Mechanism of action of a WWTR1(TAZ)-CAMTA1 fusion oncoprotein.
Tanas MR, Ma S, Jadaan FO, Ng CK, Weigelt B, Reis-Filho JS, Rubin BP
Oncogene 2016 Feb 18;35(7):929-38
Oncogene 2016 Feb 18;35(7):929-38
Integrative genomics analysis reveals the multilevel dysregulation and oncogenic characteristics of TEAD4 in gastric cancer.
Lim B, Park JL, Kim HJ, Park YK, Kim JH, Sohn HA, Noh SM, Song KS, Kim WH, Kim YS, Kim SY
Carcinogenesis 2014 May;35(5):1020-7
Carcinogenesis 2014 May;35(5):1020-7
Induction of a trophoblast-like phenotype by hydralazine in the p19 embryonic carcinoma cell line.
O'Driscoll CM, Coulter JB, Bressler JP
Biochimica et biophysica acta 2013 Mar;1833(3):460-7
Biochimica et biophysica acta 2013 Mar;1833(3):460-7
Transcription factor TEAD4 regulates expression of myogenin and the unfolded protein response genes during C2C12 cell differentiation.
Benhaddou A, Keime C, Ye T, Morlon A, Michel I, Jost B, Mengus G, Davidson I
Cell death and differentiation 2012 Feb;19(2):220-31
Cell death and differentiation 2012 Feb;19(2):220-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 over-expressed 293 cell line, cotransfected with TEAD4 Validated Chimera RNAi ( Cat # H00007004-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TEAD4 monoclonal antibody (M01), clone 5H3 (Cat # H00007004-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 expression in transfected 293T cell line by TEAD4 monoclonal antibody (M01), clone 5H3.Lane 1: TEAD4 transfected lysate(34.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TEAD4 Antibody (5H3) [H00007004-M01] - TEAD4 monoclonal antibody (M01), clone 5H3 Analysis of TEAD4 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TEAD4 Antibody (5H3) [H00007004-M01] - Detection limit for recombinant GST tagged TEAD4 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TEAD4 Antibody (5H3) [H00007004-M01] - Analysis of TEAD4 transfected lysate using anti-TEAD4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TEAD4 MaxPab rabbit polyclonal antibody.
