PA5-21977

antibody from Invitrogen Antibodies

Targeting: TEAD4 EFTR-2, RTEF-1, TCF13L1, TEF-3, TEFR-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21977

Provider

Invitrogen Antibodies

Product name

TEAD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, HepG2 nuclear extract.

Concentration

1.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TEAD4 using 20 µg of HepG2 nucleus lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TEAD4 polyclonal antibody (Product # PA5-21977) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TEAD4 was performed by separating 30 µg of HepG2 whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TEAD4 Polyclonal Antibody (Product # PA5-21977) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using TEAD4 Polyclonal Antibody (Product # PA5-21977). HepG2 whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD4 Polyclonal Antibody (Product # PA5-21977) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of TEAD4 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TEAD4 Polyclonal Antibody (Product # PA5-21977) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TEAD4 Polyclonal Antibody detects TEAD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEAD4 stained by TEAD4 Polyclonal Antibody (Product # PA5-21977) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TEAD4 (Product # PA5-21977) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.