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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX632636 - Provider product page
- Provider
- GeneTex
- Product name
- SDHA antibody [GT20710]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:400.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SDHA antibody [GT20710] detects SDHA protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. SDHA antibody [GT20710] (GTX632636) diluted at 1:250.
