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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-59087 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPON2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: HPANSFYYPR LKALPPIARV TLLRLRQSPR AFIPPAPVLP SRDNEIVDSA SVPETPLDCE VSLWSSWGLC GGHCGRLGTK SRTRYVRVQP ANNGS
- Concentration
- 0.1 mg/mL
Submitted references Expression of Extracellular Matrix-Related Genes and Their Regulatory microRNAs in Problematic Colorectal Polyps.
Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development.
Žlajpah M, Boštjančič E, Tepeš B, Zidar N
Cancers 2020 Dec 11;12(12)
Cancers 2020 Dec 11;12(12)
Hippo Signaling Plays an Essential Role in Cell State Transitions during Cardiac Fibroblast Development.
Xiao Y, Hill MC, Zhang M, Martin TJ, Morikawa Y, Wang S, Moise AR, Wythe JD, Martin JF
Developmental cell 2018 Apr 23;45(2):153-169.e6
Developmental cell 2018 Apr 23;45(2):153-169.e6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPON2 in control (vector only transfected HEK293T lysate) and SPON2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SPON2 Polyclonal Antibody (Product # PA5-59087).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPON2 in human kidney tissue shows strong cytoplasmic positivity in tubular cells. Samples were probed using a SPON2 Polyclonal Antibody (Product # PA5-59087).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ( a ) Adenoma; ( b ) adenoma with epithelial misplacement--epithelial misplacement in the submucosa is marked by asterix *; ( c ) adenoma with early carcinoma; invasive carcinoma in the submucosa is marked by asterix *. HE staining, orig. magnification 4x. EPHA4: Mild positive reaction in epithelial cells in adenoma ( d ). More intensive reaction in epithelial cells and in the stroma in epithelial misplacement ( e ). Focal reaction in carcinoma cells and in the stroma of invasive carcinoma ( f ). SPON2: Positive reaction in epithelial cells and in the stroma in adenoma ( g ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( h ). Strong reaction in carcinoma cells and positive reaction in the stroma of invasive carcinoma ( i ). SPP1: Positive reaction in epithelial cells and mild reaction in the stroma in adenoma ( j ). Positive reaction in epithelial cells and in the stroma in epithelial misplacement ( k ). Strong reaction in carcinoma cells and in the stroma of invasive carcinoma ( l ). Immunohistochemistry, orig. magnification 10x.
