27190-1-AP

antibody from Invitrogen Antibodies

Targeting: SLC9A3 NHE3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

27190-1-AP

Provider

Invitrogen Antibodies

Product name

NHE3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KSTKLGLNQ NKKAAKLYKR ERAQKRRNSS IPNGKLPMES PAQNFTIKEK DLELSDTEEP PNYDEEMSGG IEFLASVTKD TASDSPAGID NPVFSPDEAL DRSLLARLPP WLSPGETVVP SQRARTQIPY SPGTFRRLMP FRLSSKSVDS FLQADGPEER PPAALPESTH M (665-834 aa encoded by BC101669)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.57 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP ( NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 27190-1-AP ( NHE3 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney tissue slide using 27190-1-AP ( NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9). Arrows show brush border.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.