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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324380 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal CAF-1 Antibody (N-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal CAF-1 Antibody (N-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CNOT7
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.31 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CAF-1 Antibody (N-term) (Cat. #TA324380) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CAF-1 antibody detected the CAF-1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with CAF-1 Antibody (N-term)(Cat#TA324380).Hela cells were incubated with CAF-1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CAF-1 Antibody (N-term) (Cat. #TA324380)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
