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- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-49833 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49833, RRID:AB_10012023
- Product name
- Goat Polyclonal TPPP/p25 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Antigen sequence
DPSKDRAAKRLSLES - Internal region- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references An unstructured protein with destructive potential: TPPP/p25 in neurodegeneration.
The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules.
Ovádi J, Orosz F
BioEssays : news and reviews in molecular, cellular and developmental biology 2009 Jun;31(6):676-86
BioEssays : news and reviews in molecular, cellular and developmental biology 2009 Jun;31(6):676-86
The phosphorylation of p25/TPPP by LIM kinase 1 inhibits its ability to assemble microtubules.
Acevedo K, Li R, Soo P, Suryadinata R, Sarcevic B, Valova VA, Graham ME, Robinson PJ, Bernard O
Experimental cell research 2007 Dec 10;313(20):4091-106
Experimental cell research 2007 Dec 10;313(20):4091-106
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - (2ug/ml) staining of Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Negative Control showing staining of Human Cerebellum, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
