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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-19243 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPPP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with porcine based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination.
Pharmaceutical integrated stress response enhancement protects oligodendrocytes and provides a potential multiple sclerosis therapeutic.
Fu MM, McAlear TS, Nguyen H, Oses-Prieto JA, Valenzuela A, Shi RD, Perrino JJ, Huang TT, Burlingame AL, Bechstedt S, Barres BA
Cell 2019 Sep 19;179(1):132-146.e14
Cell 2019 Sep 19;179(1):132-146.e14
Pharmaceutical integrated stress response enhancement protects oligodendrocytes and provides a potential multiple sclerosis therapeutic.
Way SW, Podojil JR, Clayton BL, Zaremba A, Collins TL, Kunjamma RB, Robinson AP, Brugarolas P, Miller RH, Miller SD, Popko B
Nature communications 2015 Mar 13;6:6532
Nature communications 2015 Mar 13;6:6532
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TPPP/p25 in Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Samples were probed with the TPPP/p25 antibody (Product # PA5-19243, 0.001µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Cerebellum lysate using Product # PA5-19243 at a concentration of 0.001 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TPPP in Human Cortex using a TPPP monoclonal antibody (Product #PA5-19243) at 3.75 µg/mL. The Human Cortex tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TTP/p25 in paraffin embedded Human Cerebral Cortex tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the TTP/p25 antibody (Product # PA5-19243) (3.8µg/mL) and AP-staining.
