Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-35958 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSPC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2. Predicted reactivity: Mouse (94%), Rat (94%), Rhesus Monkey (97%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPC1 Polyclonal Antibody detects PSPC1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg HepG2 whole cell lysate/extract.7.5% SDS-PAGE. PSPC1 Polyclonal Antibody (Product # PA5-35958) dilution: 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPC1 Polyclonal Antibody detects PSPC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSPC1 protein stained by PSPC1 Polyclonal Antibody (Product # PA5-35958) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPC1 Polyclonal Antibody detects PSPC1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PSPC1 Polyclonal Antibody (Product # PA5-35958) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PSPC1 Polyclonal Antibody detects PSPC1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. PSPC1 Polyclonal Antibody (Product # PA5-35958) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.