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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM31803PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31803PU-N, RRID:AB_10978341
- Product name
- anti Necdin
- Antibody type
- Monoclonal
- Antigen
- NDN antibody was raised against nDN (NP_002478, 222 a.a. ~ 322 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B3
- Vial size
- 50 µg
- Concentration
- 0,5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- NDN monoclonal antibody LS-B6103 Western Blot analysis of NDN expression in HL-60.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody AM31803PU-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NDN is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10 µg/ml]
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
