H00004692-M02

antibody from Novus Biologicals

Targeting: NDN HsT16328, PWCR

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00004692-M02

Provider

Novus Biologicals

Proper citation

Novus Cat#H00004692-M02, RRID:AB_608894

Product name

Mouse Monoclonal Necdin Antibody

Antibody type

Monoclonal

Description

IgG purified. NDN - necdin homolog (mouse)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Notch3 protein expression in skin fibroblasts from CADASIL patients.

Qualtieri A, Ungaro C, Bagalà A, Bianchi S, Pantoni L, Moccia M, Mazzei R
Journal of the neurological sciences 2018 Jul 15;390:121-128

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Necdin enhances myoblasts survival by facilitating the degradation of the mediator of apoptosis CCAR1/CARP1.

François S, D'Orlando C, Fatone T, Touvier T, Pessina P, Meneveri R, Brunelli S
PloS one 2012;7(8):e43335

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - Analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi ( Cat # H00004692-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody (M02), clone 1B3 (Cat # H00004692-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - Analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody (M02), clone 1B3.Lane 1: NDN transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Necdin Antibody (1B3) [H00004692-M02] - NDN monoclonal antibody (M02), clone 1B3 Analysis of NDN expression in HL-60.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: Necdin Antibody (1B3) [H00004692-M02] - Detection limit for recombinant GST tagged NDN is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Necdin Antibody (1B3) [H00004692-M02] - Analysis of monoclonal antibody to NDN on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Necdin Antibody (1B3) [H00004692-M02] - Analysis of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml.