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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 11255-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD51AP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MVRPVRHKK PVNYSQFDHS DSDDDFVSAT VPLNKKSRTA PKELKQDKPK PNLNNLRKEE IPVQEKTPKK RLPEGTFSIP ASAVPCTKMA LDDKLYQRDL EVALALSVKE LPTVTTNVQN SQDKSIEKHG SSKIETMNKS PHISNCSVAS DYLDLDKITV EDDVGGVQGK RKAASKAAAQ QRKILLEGSD GDSANDTEPD FAPGEDSEDD SDFCESEDND EDFSMRKSKV KEIKKKEVKV KSPVEKKEKK SKSKCNALVT SVDSAPAAVK SESQSLPKKV SLSSDTTRKP LEIRSPSAES KKPKWVPPAA SGGSRSSSSP LVVVSVKSPN QSLRLGLSRL ARVKPLHPNA TST (1-352 aa encoded by BC016330)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
