PA5-142472

antibody from Invitrogen Antibodies

Targeting: CLEC2D CLAX, LLT1, OCIL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142472

Provider

Invitrogen Antibodies

Product name

CLEC2D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CLEC2D in U937 cell lysate (35 µg protein in RIPA buffer) using CLEC2D Polyclonal Antibody (Product # PA5-142472) at 0.3 µg/mL. Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CLEC2D in A431 cells using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of CLEC2D in paraffin embedded Human Tonsil using CLEC2D Polyclonal Antibody (Product # PA5-142472) at 3.75 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of CLEC2D in paraffin embedded Human Placenta using CLEC2D Polyclonal Antibody (Product # PA5-142472) at 3.75 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of CLEC2D in Jurkat cells (blue line) using CLEC2D Polyclonal Antibody (Product # PA5-142472). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.