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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52886PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52886PU-N, RRID:AB_11146252
- Product name
- anti NKD2 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 373-401aa) of human NKD2
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in Jurkat cell line lysates (35ug/lane) using NKD2 antibody Cat.-No. AP52886PU-N (C-term). This demonstrates this antibody detected the NKD2 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of A375 cells using NKD2 antibody Cat.-No. AP52886PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human kidney tissue (Formalin-fixed, Paraffin-embedded) using NKD2 antibody Cat.-No. AP52886PU-N (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using NKD2Â antibody Cat.-No. AP52886PU-N (C-term), followed by FITC-conjugated donkey-anti-rabbit secondary antibodies.
