PA5-61979

antibody from Invitrogen Antibodies

Targeting: NKD2 Naked2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-61979

Provider

Invitrogen Antibodies

Product name

Naked2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GHKRYRQKGR EGHSPLKAPH AQPATVEHEV VRDLPPTPAG EGYAVPVIQR HE

Concentration

1.0 mg/mL

References

Submitted references

Decoding myofibroblast origins in human kidney fibrosis.

Kuppe C, Ibrahim MM, Kranz J, Zhang X, Ziegler S, Perales-Patón J, Jansen J, Reimer KC, Smith JR, Dobie R, Wilson-Kanamori JR, Halder M, Xu Y, Kabgani N, Kaesler N, Klaus M, Gernhold L, Puelles VG, Huber TB, Boor P, Menzel S, Hoogenboezem RM, Bindels EMJ, Steffens J, Floege J, Schneider RK, Saez-Rodriguez J, Henderson NC, Kramann R
Nature 2021 Jan;589(7841):281-286

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Naked2 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a Naked2 Polyclonal Antibody (Product # PA5-61979).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Naked2 in human kidney tissue shows strong cytoplasmic positivity in subset of tubules. Samples were probed using a Naked2 Polyclonal Antibody (Product # PA5-61979).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Nkd2 as therapeutic target. a . Expression of Nkd2 visualized on the UMAP embedding from Figure 3j . b . Percent of Col1a1+/- cells in mouse Pdgfra+/Pdgfrb+ cells ( Figure 3j , stratified by Pdgfra and Nkd2 expression). c . Scaled gene expression of Nkd2 correlating or anti-correlating genes in human Pdgfrb+ cells ( Figure 2 ). d.-e . RNA in-situ hybridization (ISH) of PDGFRa, PDGFRb and NKD2 in human kidneys and quantification of triple positive cells (n=36, Patient data in Extended Data Table 2). n=20 and 16. Two-tailed Mann-Whitney test. Tukey box whisker plot. IF-score = interstitial fibrosis score. Scale bar 10mum. f.-g . Representative Western blots of Nkd2 overexpression and KO cells. For gel source data, see Extended Data Fig. 10e . h . GSEA (Gene set enrichment analysis) of ECM genes in Nkd2-perturbed PDGFRb - kidney cells. n=3 each. * P < 0.05, **p< 0.01, and ***p < 0.001 as determined by FGSEA-multilevel method after adjusting p-values for multiple testing correction (Benjamini & Hochberg). i . ISH of Pdgfra, Pdgfrb and Nkd2 in human iPSC derived kidney organoids. j . Quantification of Nkd2 RNA expression in organoids. n=4 each. Two-tailed unpaired t-test. k.-l . Immunofluorescence staining and quantification of Col1a1 in organoids. n=4 each. * P < 0.05, **p< 0.01, and ***p < 0.001 by 1-way ANOVA followed by Bonferroni's correction. Scale bar in i+k 50 mum. Data shown as mean+-SD. For details on statistics and reproducibility, please see Methods .