Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN953712 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Naked Cuticle Homolog 2 (Drosophila) (NKD2) (AA 373-401), (C-Term) antibody
- Antibody type
- Polyclonal
- Description
- Purified through a Protein A column followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 373-401, C-Term
- Vial size
- 0.4 mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane.
EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation.
Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells.
Frequent amplifications and abundant expression of TRIO, NKD2, and IRX2 in soft tissue sarcomas.
Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells.
Hu T, Li C, Cao Z, Van Raay TJ, Smith JG, Willert K, Solnica-Krezel L, Coffey RJ
The Journal of biological chemistry 2010 Apr 30;285(18):13561-8
The Journal of biological chemistry 2010 Apr 30;285(18):13561-8
EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation.
Ding W, Li C, Hu T, Graves-Deal R, Fotia AB, Weissman AM, Coffey RJ
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 9;105(36):13433-8
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 9;105(36):13433-8
Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells.
Li C, Hao M, Cao Z, Ding W, Graves-Deal R, Hu J, Piston DW, Coffey RJ
Molecular biology of the cell 2007 Aug;18(8):3081-93
Molecular biology of the cell 2007 Aug;18(8):3081-93
Frequent amplifications and abundant expression of TRIO, NKD2, and IRX2 in soft tissue sarcomas.
Adamowicz M, Radlwimmer B, Rieker RJ, Mertens D, Schwarzbach M, Schraml P, Benner A, Lichter P, Mechtersheimer G, Joos S
Genes, chromosomes & cancer 2006 Sep;45(9):829-38
Genes, chromosomes & cancer 2006 Sep;45(9):829-38
Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells.
Li C, Franklin JL, Graves-Deal R, Jerome WG, Cao Z, Coffey RJ
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 13;101(15):5571-6
Proceedings of the National Academy of Sciences of the United States of America 2004 Apr 13;101(15):5571-6
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of A375 cells using NKD2 antibody Cat.-No. AP52886PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS