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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-48378 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP5J Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with monkey based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5J in mouse stomach tissue lysates (35 µg/lane). Samples were probed with a ATP5J Antibody (Center) (Product # PA5-48378).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP5J in rat heart tissue. Samples were probed with a ATP5J polyclonal antibody (Product # PA5-48378) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 µg.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ATP5J in U251 cells. Cells were fixed, permeabilized and incubated with a ATP5J polyclonal antibody (Product # PA5-48378) diluted at 1:25. Following incubation with the primary antibody for 1 hour at 37°C, cells were stained with an Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). ATP5J immunoreactivity is localized to Mitochondria significantly.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ATP5J in formalin fixed and paraffin embedded human liver tissue. Samples were probed with a ATP5J Antibody (Center) (Product # PA5-48378) followed by peroxidase conjugation of the secondary antibody and DAB staining.
