14114-1-AP

antibody from Invitrogen Antibodies

Targeting: ATP5PF ATP5, ATP5A, ATP5J, ATPM, CF6

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14114-1-AP

Provider

Invitrogen Antibodies

Product name

ATP5J Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MILQRLFRF SSVIRSAVSV HLRRNIGVTA VAFNKELDPI QKLFVDKIRE YKSKRQTSGG PVDASSEYQQ ELERELFKLK QMFGNADMNT FHTFKFEDPK FEVIEKPQA (1-108 aa encoded by BC066310 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 14114-1-AP (ATP5J antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human osteosarcoma using 14114-1-AP (ATP5J antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ATP5J (IP:14114-1-AP, 4ug; Detection:14114-1-AP 1:300) with HEK-293 cells lysate 3680ug.