50-9774-42

antibody from Invitrogen Antibodies

Targeting: GZMM LMET1, MET1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

50-9774-42

Provider

Invitrogen Antibodies

Product name

Granzyme M Monoclonal Antibody (4B2G4), eFluor™ 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: Granzyme M is one of five granzyme serine proteases that have been identified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Although not as well characterized as some of the other family members, Granzyme M has been found to exhibit some distinct functions. These include the non-cytotoxic inhibition of cytomegalovirus replication, induction of apoptosis in tumor cells, and contribution to the TLR4-induced inflammatory process.

Antibody clone number

4B2G4

Concentration

5 µL/Test

References

Submitted references

CD8(+) T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity.

Nguyen THO, Rowntree LC, Petersen J, Chua BY, Hensen L, Kedzierski L, van de Sandt CE, Chaurasia P, Tan HX, Habel JR, Zhang W, Allen LF, Earnest L, Mak KY, Juno JA, Wragg K, Mordant FL, Amanat F, Krammer F, Mifsud NA, Doolan DL, Flanagan KL, Sonda S, Kaur J, Wakim LM, Westall GP, James F, Mouhtouris E, Gordon CL, Holmes NE, Smibert OC, Trubiano JA, Cheng AC, Harcourt P, Clifton P, Crawford JC, Thomas PG, Wheatley AK, Kent SJ, Rossjohn J, Torresi J, Kedzierska K
Immunity 2021 May 11;54(5):1066-1082.e5

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Human γδ T-cell receptor repertoire is shaped by influenza viruses, age and tissue compartmentalisation.

Sant S, Jenkins MR, Dash P, Watson KA, Wang Z, Pizzolla A, Koutsakos M, Nguyen TH, Lappas M, Crowe J, Loudovaris T, Mannering SI, Westall GP, Kotsimbos TC, Cheng AC, Wakim L, Doherty PC, Thomas PG, Loh L, Kedzierska K
Clinical & translational immunology 2019;8(9):e1079

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Resistance of HIV-infected macrophages to CD8(+) T lymphocyte-mediated killing drives activation of the immune system.

Clayton KL, Collins DR, Lengieza J, Ghebremichael M, Dotiwala F, Lieberman J, Walker BD
Nature immunology 2018 May;19(5):475-486

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Granzyme M targets host cell hnRNP K that is essential for human cytomegalovirus replication.

van Domselaar R, de Poot SA, Remmerswaal EB, Lai KW, ten Berge IJ, Bovenschen N
Cell death and differentiation 2013 Mar;20(3):419-29

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Human and mouse granzyme M display divergent and species-specific substrate specificities.

de Poot SA, Westgeest M, Hostetter DR, Van Damme P, Plasman K, Demeyer K, Broekhuizen R, Gevaert K, Craik CS, Bovenschen N
The Biochemical journal 2011 Aug 1;437(3):431-42

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A role for granzyme M in TLR4-driven inflammation and endotoxicosis.

Anthony DA, Andrews DM, Chow M, Watt SV, House C, Akira S, Bird PI, Trapani JA, Smyth MJ
Journal of immunology (Baltimore, Md. : 1950) 2010 Aug 1;185(3):1794-803

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells were stimulated for 3 days with Human IL-2 Recombinant Protein (Product # 14-8029-81), then cultured with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) for an additional 5 hours. The cells were intracellularly stained with Anti-Human CD8 FITC (Product # 11-0087-42) and Mouse IgG1 Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human Granzyme M eFluor® 660 (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.