PA5-109279

antibody from Invitrogen Antibodies

Targeting: KCNA5 HK2, HPCN1, Kv1.5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109279

Provider

Invitrogen Antibodies

Product name

KV1.5 (KCNA5) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: PANC-1, LOVO, SKOV-3, rat skeletal muscle tissue, rat heart tissue.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KCNA5 in PANC-1 cell lysate. Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279), at a dilution of 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KCNA5 in SKOV-3 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KCNA5 in PANC-1 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KCNA5 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KCNA5 polyclonal antibody (Product # PA5-109279), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KCNA5 in paraffin-embedded rat skeletal muscle tissue. Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KCNA5 in paraffin-embedded rat heart tissue. Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of KCNA5 in PANC-1 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of KCNA5 in PANC-1 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KCNA5 polyclonal antibody (Product # PA5-109279) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.