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LearnNB100-2475
antibody from Novus Biologicals
Targeting: IFT88
D13S1056E, hTg737, MGC26259, Tg737, TTC10
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-2475 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2475, RRID:AB_2264482
- Product name
- Goat Polyclonal IFT88 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_783195.2
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references CLEM Methods for Studying Primary Cilia.
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.
News focus: Betting on cilia.
Macaluso FP, Perumal GS, Kolstrup J, Satir P
Methods in molecular biology (Clifton, N.J.) 2016;1454:193-202
Methods in molecular biology (Clifton, N.J.) 2016;1454:193-202
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
Miyoshi K, Kasahara K, Miyazaki I, Shimizu S, Taniguchi M, Matsuzaki S, Tohyama M, Asanuma M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Oct;23(10):3289-97
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Oct;23(10):3289-97
Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.
Jiang ST, Chiou YY, Wang E, Chien YL, Ho HH, Tsai FJ, Lin CY, Tsai SP, Li H
Human molecular genetics 2009 May 1;18(9):1566-77
Human molecular genetics 2009 May 1;18(9):1566-77
News focus: Betting on cilia.
Vogel G
Science (New York, N.Y.) 2005 Oct 14;310(5746):216-8
Science (New York, N.Y.) 2005 Oct 14;310(5746):216-8
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFT88 Antibody [NB100-2475] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFT88 Antibody [NB100-2475] - (0.5ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IFT88 Antibody [NB100-2475] - Staining of A549 (A) cell lysate and Mouse Testes (B) lysate (35 ug protein in RIPA buffer). Antibody at 3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IFT88 Antibody [NB100-2475] - Human Kidney using this antibody at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IFT88 Antibody [NB100-2475] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
