PA1-18385

antibody from Invitrogen Antibodies

Targeting: NTF3 NGF2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-18385

Provider

Invitrogen Antibodies

Product name

NTF3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute in 20 µL of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

Reactivity

Human, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

20 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

References

Submitted references

Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.

Auer TO, Xiao T, Bercier V, Gebhardt C, Duroure K, Concordet JP, Wyart C, Suster M, Kawakami K, Wittbrodt J, Baier H, Del Bene F
eLife 2015 Jun 15;4

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6. Expression of the neurotrophic factor neurotrophin 3 in visually impaired mutants. ( A ) Relative expression levels of bdnf , ntf3, ntf4, ntf7 , and ngf in 4 dpf old wild-type and kif5aa mutant embryos. ntf3 is upregulated to 160% of wild-type expression levels (p < 0.05) while all other neurotrophic factors show the same expression levels between wild-type and mutant embryos. ( B ) Transversal sections through the tectum after in situ hybridization with an ntf3 specific antisense probe detect higher expression levels of ntf3 in tecta of kif5aa . Scale bars = 25 mum. PC = pigment cell, NP = neuropil, PVN = periventricular neurons. Asterisk = strong ntf3 signal in the otic vesicle. ( C ) Confirmation of Ntf3 overexpression by Western blotting in 4 dpf old embryos. To show the specificity of the antibody, we generated a Ntf3 overexpression construct (UAS:ntf3-E2A-RFP). The two visually impaired mutant lines lakritz and blumenkohl also show a substantial upregulation of ntf3 expression levels. ( D ) Quantification of Ntf3 protein expression levels based on Western blotting data in wild-type and visually impaired mutant embryos. All three mutant lines show a substantial upregulation of Ntf3 protein levels. DOI: http://dx.doi.org/ Figure 6--figure supplement 1. Silencing of all Retinal Ganglion Cells by BoTx expression leads to Ntf3 upregulation. ( A ) Expression of botulinum toxin light chain B ( Brunger et al., 2008 ; Nevin et al., 2008 ) in all RGCs leads to expansion

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6--figure supplement 1. Silencing of all Retinal Ganglion Cells by BoTx expression leads to Ntf3 upregulation. ( A ) Expression of botulinum toxin light chain B ( Brunger et al., 2008 ; Nevin et al., 2008 ) in all RGCs leads to expansion of melanosomes similar to blind mutant fish as shown in Figure 1--figure supplement 1 . Scale bar = 200 mum. ( B ) Relative expression levels of ntf3 in 4-dpf old wildtype, kif5aa mutant, and Tg(Isl2b:Gal4, UAS:BoTxLCB-GFP) transgenic embryos. ntf3 is upregulated to a similar extent in Tg(Isl2b:Gal4, UAS:BoTxLCB-GFP) transgenic embryos as in kif5aa mutants. ( C ) Western blotting confirms the overexpression of Ntf3 protein in embryos without presynaptic activity. DOI: http://dx.doi.org/