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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 18084-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Neurotrophin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSILFYVIF LAYLRGIQGN NMDQRSLPED SLNSLIIKLI QADILKNKLS KQMVDVKENY QSTLPKAEAP REPERGGPAK SAFQPVIAMD TELLRQQRRY NSPRVLLSDS TPLEPPPLYL MEDYVGSPVV ANRTSRRKRY AEHKSHRGEY SVCDSESLWV TDKSSAIDIR GHQVTVLGEI KTGNSPVKQY FYETRCKEAR PVKNGCRGID DKHWNSQCKT SQTYVRALTS ENNKLVGWRW IRIDTSCVCA LSRKIGRT (1-257 aa encoded by BC069773)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 18084-1-AP (Neurotrophin 3 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Neurotrophin 3 (IP: 18084-1-AP, 4 µg; Detection: 18084-1-AP 1:800) with SH-SY5Y cells lysate 1600 µg.
