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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-23946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL17D Monoclonal Antibody (246018)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, rhIL-17E, or rhIL-17F is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 246018
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A short deletion in the DNA-binding domain of STAT3 suppresses growth and progression of colon cancer cells.
Xiong YJ, Liu DY, Shen RR, Xiong Y
Aging 2021 Feb 1;13(4):5185-5196
Aging 2021 Feb 1;13(4):5185-5196
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IL-17D was detected in immersion fixed paraffin-embedded sections of human heart using 25 µg/mL human IL-17D Monoclonal Antibody (Product # MA5-23946) overnight at 4 °C. Tissue was stained with the Anti-mouse HRP-DAB (brown and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human peripheral blood mononuclear cells were stained with human IL-17D Monoclonal Antibody (Product # MA5-23946) or isotype control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 STAT3 del reduces in vitro growth, survival and migration of colon cancer cells. ( A ) CCK8 assay results show the proliferation rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . ( B ) TUNEL assay results show the apoptotic rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . ( C ) Wound healing assay results show the migration rate of SW480, SW620 and HCT-116 cells with wild type STAT3 or STAT3 del . * denotes p
