- MA5-13490 - Invitrogen Antibodies PVR antibody

Submitted references Physical Characterization of Colorectal Cancer Spheroids and Evaluation of NK Cell Infiltration Through a Flow-Based Analysis.

Sargenti A, Musmeci F, Bacchi F, Delprete C, Cristaldi DA, Cannas F, Bonetti S, Pasqua S, Gazzola D, Costa D, Villa F, Zocchi MR, Poggi A
Frontiers in immunology 2020;11:564887

Inhibition of Necl-5 (CD155/PVR) reduces glioblastoma dispersal and decreases MMP-2 expression and activity.

Enloe BM, Jay DG
Journal of neuro-oncology 2011 Apr;102(2):225-35

NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.

Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25

CD155/PVR enhances glioma cell dispersal by regulating adhesion signaling and focal adhesion dynamics.

Sloan KE, Stewart JK, Treloar AF, Matthews RT, Jay DG
Cancer research 2005 Dec 1;65(23):10930-7

DNAM-1 and PVR regulate monocyte migration through endothelial junctions.

Reymond N, Imbert AM, Devilard E, Fabre S, Chabannon C, Xerri L, Farnarier C, Cantoni C, Bottino C, Moretta A, Dubreuil P, Lopez M
The Journal of experimental medicine 2004 May 17;199(10):1331-41

Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.

Ohka S, Matsuda N, Tohyama K, Oda T, Morikawa M, Kuge S, Nomoto A
Journal of virology 2004 Jul;78(13):7186-98

Identification of secreted CD155 isoforms.

Baury B, Masson D, McDermott BM Jr, Jarry A, Blottière HM, Blanchardie P, Laboisse CL, Lustenberger P, Racaniello VR, Denis MG
Biochemical and biophysical research communications 2003 Sep 12;309(1):175-82

Identification of secreted CD155 isoforms.

Baury B, Masson D, McDermott BM Jr, Jarry A, Blottière HM, Blanchardie P, Laboisse CL, Lustenberger P, Racaniello VR, Denis MG
Biochemical and biophysical research communications 2003 Sep 12;309(1):175-82

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD155 was performed by loading 25 µg of mouse stomach (Lane 1), U-937 (Lane 2) and Hela (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 45 kDa. This image was obtained using the unconjugated form of the antibody.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD155 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD155 monoclonal antibody (Product # MA5-13493) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD155 (green) showing staining in the membrane of U-87 MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD155 monoclonal antibody (Product # MA5-13493) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD155 in U937 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD155 monoclonal antibody (Product # MA5-13493) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

MA5-13490

Antibody data