Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- RQ6433 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Proteasome subunit beta type-5 Antibody / PSMB5 / MB1
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Proteasome subunit beta type-5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human HepG2, 3) human A549, 4) rat liver, 5) rat pancreas, 6) rat testis, 7) rat kidney, 8) mouse liver, 9) mouse testis and 10) mouse kidney tissue lysate with Proteasome subunit beta type-5 antibody. Predicted molecular weight: 18-28 kDa (multiple isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human MCF7 cells with Proteasome subunit beta type-5 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lymphoma tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer tissue with Proteasome subunit beta type-5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with Proteasome subunit beta type-5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Proteasome subunit beta type-5 antibody.